Brain-derived extracellular vesicle microRNAs in Lewy body and Alzheimer's disease.

ABSTRACT:

Introduction

Robust plasma-based biomarkers to distinguish Lewy body disease (LBD) and Alzheimer's disease (AD) are currently lacking. We applied track-etch magnetic nanopore (TENPO) sorting for enrichment of brain-derived extracellular vesicle (EV) signatures as potential biomarkers to address this gap.

Methods

We analyzed plasma from 137 autopsy-confirmed patients [30 LBD, 31 AD, 30 AD/LBD, 19 AD with amygdala Lewy bodies (AD/ALB), and 27 controls], sequencing miRNAs from TENPO-isolated GluR2-positive (neuron-enriched) and GLAST-positive (astrocyte-enriched) EVs, and measuring plasma proteins (Aβ40, Aβ42, tau, p-Tau181, p-Tau231) via SIMOA.

Results

We identified 16 GluR2+, 8 GLAST+, and 4 protein biomarkers with differential expression (false discovery rate-correcte

SUBMITTER: Yang SJ 

PROVIDER: S-EPMC12258949 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature