Multiphasic Organization and Differential Dynamics of Proteins Within Protein-DNA Biomolecular Condensates.

ABSTRACT: Biomolecular condensates formed through liquid-liquid phase separation are increasingly recognized as critical regulators of genome organization and gene expression. While the role of proteins in driving phase separation is well-established, how DNA modulates the structure and dynamics of protein-DNA condensates remains less well understood. Here, we employ a minimalist coarse-grained model to investigate the interplay between homotypic protein-protein and heterotypic protein-DNA interactions in governing condensate formation, composition, and internal dynamics. Our simulations reveal that DNA chain length and flexibility critically influence condensate morphology, leading to the emergence of multiphasic and core-shell organizations under strong heterotypic interactions. We find that DNA r

SUBMITTER: Tangade AS 

PROVIDER: S-EPMC12259059 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature