Depth-Resolved Macroscopic Fluorescence Lifetime Imaging via High-Spatial-Frequency Structured Illumination.

ABSTRACT:

Significance

Macroscopic Fluorescence Lifetime Imaging (MFLI) is a powerful, non-invasive imaging modality that offers robust, physiologically relevant contrast largely independent of fluorophore concentration, excitation intensity, and tissue signal attenuation. However, accurately determining the depth of signal origin remains challenging, potentially leading to ambiguity in biological interpretation. Here, we present a novel optical correction method that effectively eliminates surface signal bias, such as that from skin in preclinical imaging, without the need for chemical clearance. This advancement supports the robust applicability of MFLI in translational research.

Aim

Establishment of a High Spatial Frequency-Fluorescence Lifetime Imaging (HSF-FLI) framework to selec

SUBMITTER: Yuan N 

PROVIDER: S-EPMC12259142 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature