Synthetic Rewiring of Virus-Like Particles via Circular Permutation Enables Modular Peptide Display and Protein Encapsulation.

ABSTRACT: Virus-like particles (VLPs) are self-assembling nanoparticles derived from viruses with potential as scaffolds for myriad applications. They are also excellent testbeds for engineering protein superstructures. Engineers often employ techniques such as amino acid substitutions and insertions/deletions. Yet evolution also utilizes circular permutation, a powerful natural strategy that has not been fully explored in engineering self-assembling protein nanoparticles. Here, we demonstrate this technique using the MS2 VLP as a model self-assembling, proteinaceous nanoparticle. We constructed, for the first time, a comprehensive circular permutation library of the fused MS2 coat protein dimer construct. The strategy revealed new terminal locations, validated via cryo-electron microscopy, that ena

SUBMITTER: Liang S 

PROVIDER: S-EPMC12259173 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature