A Multimodal Adaptive Optical Microscope For <i>In Vivo</i> Imaging from Molecules to Organisms.

ABSTRACT: Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native microenvironments. Yet, achieving this comprehensive view is challenging because microscopes optimized for specific tasks typically lack versatility due to inherent optical and sample handling trade-offs, and frequently suffer performance degradation from sample-induced optical aberrations in multicellular contexts. Here, we present MOSAIC, a reconfigurable microscope that integrates multiple advanced imaging techniques including light-sheet, label-free, super-resolution, and multi-photon, all equipped with adaptive optics. MOSAIC enables non-invasive ima

SUBMITTER: Fu TM 

PROVIDER: S-EPMC12259214 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature