GALNT6 associated with O-GlcNAcylation contributes to the tumorigenesis of oral squamous cell carcinoma.

ABSTRACT:

Background

We aimed to explore the regulatory gene associated with O-GlcNAcylation in oral squamous cell carcinoma (OSCC) and the underlying mechanism.

Methods

The publicly available gene expression datasets (GSE23558 and GSE25099) were downloaded from Gene Expression Omnibus database. Differentially expressed genes (DEGs) between tumor and controls were identified by limma package. O-GlcNAcylation related genes with differential expression were screened and filtered by LASSO, RF and SVM machine learning models. The expression of one key gene (GALNT6) was validated in OSCC cells by qRT-PCR assay. Immunohistochemistry staining was used to detect the expression of GALNT6 in clinical tissue samples. GALNT6 expression was knocked down by siRNA transfection. CCK8, cell clone, cel

SUBMITTER: Yan J 

PROVIDER: S-EPMC12259505 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature