Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.

ABSTRACT: Severe sepsis can escalate to acute lung injury (ALI), a critical condition with limited treatment options. Emerging research highlights the role of non-coding RNAs, including microRNAs (miRNAs) and circular RNAs (circRNAs), in regulating epithelial and immune cell responses in sepsis-induced ALI. This study delves into the circAGFG1/miR-195-5p/PD-L1 axis to elucidate its potential in this context. The conducted experiments using Th17 cells differentiated from CD4+ T cells isolated from human umbilical cord blood, co-cultured with transfected Calu-3 cells. The assessed cell viability, RNA, and protein expression levels under inflammatory stimuli. In addition, we collected bronchoalveolar lavage fluid and lung tissues from a cecal ligation and puncture mouse model. Quantification of RNA and

SUBMITTER: Bi Y 

PROVIDER: S-EPMC12259802 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature