Discrimination of Klebsiella pneumoniae and Klebsiella quasipneumoniae by MALDI-TOF Mass Spectrometry Coupled With Machine Learning.

ABSTRACT: Klebsiella species, including Klebsiella pneumoniae and Klebsiella quasipneumoniae, present significant challenges in clinical microbiology due to their genetic similarity, which complicates accurate species identification using established methods, including matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) on the protein/peptide level. Although the treatment choice for infections caused by these pathogens is often similar, precise species characterization enhances our epidemiological understanding. While whole-genome sequencing can accurately distinguish Klebsiella species accurately, those analyses are time-consuming, requiring specialized expertise, and are not currently used in routine clinical laboratories. Therefore, developing a timely and

SUBMITTER: Nishikawa M 

PROVIDER: S-EPMC12260210 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature