How reliable is mitochondrial DNA for recovering the phylogeny of Chironomidae (Culicomorpha: Diptera)?

ABSTRACT: We examine mitochondrial DNA as a source of data to estimate phylogenetic relationships within the Chironomidae (Diptera). Previous studies have shown that mitogenomes often produce ambiguous phylogenetic topologies that are inconsistent with both morphological and multi-locus molecular data. In this study, we sequenced 18 new mitogenomes representing 5 subfamilies, including the first available sequence for Protanypodinae. These were combined with 65 previously annotated chironomids mitogenomes, 8 additional individuals assembled from SRA data, and 8 outgroup taxa. Phylogenetic reconstructions were performed using complete protein-coding genes (PCGs), the first and second codon positions of PCGs (PCG 12), with and without ribosomal genes (12S rDNA and 16S rDNA), and amino acid sequences (

SUBMITTER: Semenchenko AA 

PROVIDER: S-EPMC12260381 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature