Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP₃R2-mediated Ca²⁺ signaling in mice.

ABSTRACT: Local neural activation drives regional increase of cerebral blood flow (CBF), in a phenomenon known as functional hyperemia. Astrocytes, which enwrap cerebral blood vessels and respond to neuronal activity through their G protein-coupled receptors (GPCRs), play a vital role in brain energy metabolism. Although astrocytic calcium (Ca²⁺) signaling has been widely studied in relation to neurovascular coupling, the role of cyclic adenosine monophosphate (cAMP), another key second messenger of GPCRs, on CBF has not been established. In this study, we explored the effects of optogenetically induced astrocytic cAMP elevation on CBF. We engineered adeno-associated viral vectors (AAVs) to express a bacterial photoactivated adenylyl cyclase in astrocytes, which triggers an increase in cA

SUBMITTER: Vittani M 

PROVIDER: S-EPMC12260423 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature