Development of a 3D-3 co-culture microbead consisting of cancer-associated fibroblasts and human umbilical vein endothelial cells for the anti-tumor drug assessment of lung cancer.

ABSTRACT:

Background

Drug resistance is a major challenge in the treatment of lung cancer. Increasing evidence indicates that the tumor microenvironment (TME), including cancer-associated fibroblasts (CAFs) and tumor endothelial cells (TECs), plays a significant role in determining the tumor sensitivity to therapeutic agents. There is, however, a dearth of models, two-dimensional (2D) or three-dimensional (3D), that represent the in vivo interaction of tumor and stromal components in the TME. The purpose of this study was to use conditionally reprogrammed lung cancer cells (CRLCs), and then use sodium alginate (Alg) and hyaluronic acid (HA) as a hydrogel matrix to establish an in vitro 3D model for co-culture of CRLCs, CAFs, and human umbilical vein endothelial cells (HUVECs).

SUBMITTER: Chen S 

PROVIDER: S-EPMC12261251 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature