A comparative study of biostatistical pipelines for benchmark concentration modeling of <i>in vitro</i> screening assays.

ABSTRACT: New approach methods (NAMs) have been prioritized to reduce the use of animals for chemical safety assessment while continuing to protect human health and the environment. A key challenge of generating toxicity data is the implementation of a standardized analysis approach for transparent and reproducible benchmark concentration (BMC) estimation and uncertainty quantification for assay developers, regulators, and other stakeholders. In this study, we compared the bioactivity results of 321 chemical samples from four established BMC analysis pipelines used for evaluation of developmental neurotoxicity (DNT) NAMs data: the ToxCast pipeline (tcpl), CRStats, DNT DIVER (Curvep and Hill pipelines). We found an overall activity hit call concordance of 77.2% and highly correlated BMC estimations (

SUBMITTER: Carstens KE 

PROVIDER: S-EPMC12261583 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature