Transcriptome analysis reveals the sex-switching mechanism of juvenile hermaphroditism in silver pomfret (Pampus argenteus).

ABSTRACT:

Background

Our previous study on silver pomfret (Pampus argenteus) demonstrated that all gonads initially develop into ovaries by 60 days post-hatch (dph). Between 80 and 120 dph, some oocytes undergo apoptosis, resulting in the development of testes and a transient hermaphroditic stage. This observation indicates a complex molecular mechanism underlying sex differentiation in this species.

Methods

Gonadal samples were collected at 90 dph, 120 dph, and 150 dph, with sex identification performed by HE staining and transcriptome sequencing. Morphological traits, including body length and weight, were measured to evaluate sexual size dimorphism. Candidate genes related to with sex differentiation were identified through differential gene expression analysis and feature selectio

SUBMITTER: Li Y 

PROVIDER: S-EPMC12261592 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature