Non-monotonic response of macrophages to mechanical stretch impacts skin wound healing.

ABSTRACT:

Background

The skin is subjected to constant mechanical stress in both healthy and wounded states. Macrophages play crucial roles in skin homeostasis and in all stages of the wound healing process. However, the effects of static mechanical stretch (MS) on macrophages and the subsequent consequences on skin cells remain largely unclear.

Methods

We applied static MS at amplitudes of 7%, 15%, and 21% to macrophages derived from THP-1 using a customized cell-stretching device, thoroughly investigating its impacts on viability, polarization, secretome, and underlying signaling pathways. Recognizing the substantial influence of the macrophage secretome on neighboring cells, we collected conditioned medium from macrophages exposed to MS (MS-CM) and evaluated its effects on keratino

SUBMITTER: Wei Q 

PROVIDER: S-EPMC12261726 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature