Rapid and specific immunoPET imaging of Nectin-4 in gastric cancer and non-small cell lung cancer using [⁶⁴Cu]Cu-NOTA-EV-F(ab')₂.

ABSTRACT:

Purpose

This study aimed to develop and evaluate [⁶⁴Cu]Cu-NOTA-EV-F(ab')₂ as a rapid and specific immunoPET imaging probe targeting Nectin-4 in gastric cancer (GC) and non-small cell lung cancer (NSCLC).

Materials and methods

F(ab')₂ fragments were generated from enfortumab vedotin (EV) using IdeS protease and conjugated with p-SCN-Bn-NOTA for radiolabeling with ⁶⁴CuCl₂. The radiochemical yield was 85.40 ± 2.43% (n = 5). In vitro binding affinity and specificity were assessed via flow cytometry and cell uptake assays using Nectin-4-positive (NCI-N87, H1975) and Nectin-4-low (HGC-27, H520) cell lines. In vivo PET imaging and biodistribution studies were conducted in murine models of GC and NSCLC to evaluate tumor targetin

SUBMITTER: Huang W 

PROVIDER: S-EPMC12261916 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature