Dual-view microscopy of single-molecule dipole orientations.

ABSTRACT: Measuring dipole orientations of fluorescent probes offers unique local structural insights of labelled biomolecules and has seen expanding applications in structural biology studies. Here we propose an alternative imaging geometry, 'dual-view' microscopy, for single-molecule dipole orientation measurements. We develop a protocol capable of simultaneously measuring absorption and emission dipole orientations of single emitters. Further, through simulation, we demonstrate that absorption dipole orientation can be accurately measured with high and uniform precision in three dimensions, significantly outperforming epifluorescence microscopy. Meanwhile the emission dipole is independently narrowed down to four possible orientations and can be uniquely determined with the co-estimated ab

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12262234 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature