ER tethering and active transport govern condensate diffusion during hyperosmotic stress.

ABSTRACT:

Background

Hyperosmotic shock and the resulting cell volume compression are commonly experienced by organs such as the kidneys, causing rapid formation of hyperosmotic phase separation (HOPS) condensates in the cytoplasm and nucleoplasm. Although the tight relationship between hyperosmotic shock and condensation has been characterized, the dynamics of biomolecular condensates in hyperosmotically compressed cells and their regulatory mechanisms remain largely unknown.

Results

We used live-cell single-particle tracking (SPT) across different time scales to systematically characterize the dynamics of HOPS condensates formed by model protein mRNA decapping enzyme 1A (DCP1A). We found that HOPS condensates predominantly exhibited sub-diffusion rather than free diffusion, whereas

SUBMITTER: Halder B 

PROVIDER: S-EPMC12262303 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature