Donor Age Impairs Vasculogenic Potential of hiPSC-Derived Endothelial Progenitors.

ABSTRACT:

Background

Human induced pluripotent stem cells (hiPSCs) hold promise for vascular regeneration, but preliminary research often relies on neonatal donors, whereas clinical applications will use cells derived from older adults. Although the impact of donor age on reprogramming efficiency has been studied, its effect on the functionality of hiPSC-derived endothelial progenitors (hiPSC-EPs) remains unclear. This question is the focus of the current study.

Methods and results

We derived EPs from iPSCs sourced from three neonatal donors (ND) and three mature donors (MD) matched 1:1 for sex and somatic cell origin. We assessed their functional, epigenetic, and transcriptomic characteristics. Despite higher CD34⁺ yields from MD-iPSCs, MD-hiPSC-EPs formed poorly interconn

SUBMITTER: Larsen B 

PROVIDER: S-EPMC12262358 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature