Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering.

ABSTRACT: Advanced genome editing technologies have enabled rapid and flexible rewriting of the Escherichia coli genome, benefiting fundamental biology and biomanufacturing. Unfortunately, some of the most useful technologies to advance genome editing in E. coli have not yet been ported into other bacterial species. For instance, the addition of bacterial retrons to the genome editing toolbox has increased the efficiency of recombineering in E. coli by enabling sustained, abundant production of ssDNA recombineering donors by reverse transcription that install flexible, precise edits in the prokaryotic chromosome. To extend the utility of this technology beyond E. coli, we surveyed the portability and versatility of retron-mediated recombineering across three different bac

SUBMITTER: Gonzalez-Delgado A 

PROVIDER: S-EPMC12262390 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature