Control of 3' Splice Site Selection in <i>S. cerevisiae</i> by a Highly Conserved Amino Acid within the Prp8 α-finger Domain.

ABSTRACT: Precise recognition of the boundaries between exons and introns (splice sites, SS) is essential for the fidelity of gene expression. In contrast with the 5'SS, the consensus 3'SS sequence in both S. cerevisiae and humans is just three nucleotides long: YAG. How the correct 3'SS is chosen among many possible alternates by the spliceosome is often unclear but likely involves proofreading by the Prp22 ATPase. In cryo-EM structures of spliceosome product (P) complexes, Glutamine 1594 in the highly conserved α-finger domain of the Prp8 protein interacts directly with the -3 pyrimidine of the 3'SS. To investigate the role of this interaction, we constructed a Prp8^Q1594A mutant and studied the impact on splicing and 3'SS selection. Using splicing reporter assays and RNA-seq, we

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12262433 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature