A scalable, all-optical method for mapping synaptic connectivity with cell-type specificity.

ABSTRACT: Single-cell transcriptomics has uncovered the enormous heterogeneity of cell types that compose each region of the mammalian brain, but describing how such diverse types connect to form functional circuits has remained challenging. Current methods for measuring the probability and strength of cell-type specific connectivity motifs principally rely on low-throughput whole-cell recording approaches. The recent development of optical tools for perturbing and observing neural circuit activity, now notably including genetically encoded voltage indicators, presents an exciting opportunity to employ optical methods to greatly increase the throughput with which circuit connectivity can be mapped physiologically. At the same time, advances in spatial transcriptomics now enable the identification of

SUBMITTER: Moya MV 

PROVIDER: S-EPMC12262474 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature