The Effects of Hypothermic Storage on Passaged Chondrocyte Viability and Redifferentiation Potential.

ABSTRACT: Cell-based transplantation therapies, such as autologous chondrocyte implantation (ACI), are used to treat focal cartilage defects caused by trauma or degeneration. In ACI, chondrocytes are isolated from non-load-bearing regions of healthy cartilage regions and then sent to a cell manufacturing laboratory, where they are expanded for cell number in monolayer culture. Once a large number of cells are obtained, they are transported to the clinic for reimplantation into the defect site. The storage and transport conditions from cell manufacturing to implantation may be a critical time that could influence cell viability and redifferentiation potential. Although hypothermic storage at sub-physiological temperatures is commonly used to preserve cell viability, long-term storage of cartilage und

SUBMITTER: Rayat D 

PROVIDER: S-EPMC12262539 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature