Super-resolution microscopy reveals distinct epigenetic states regulated by estrogen receptor activity.

ABSTRACT: Changes in gene expression regulated by ligand-dependent transcription factors such as estrogen receptor-α (ERα) involves the recruitment of coactivators including p300 that acetylates histone H3 at lysine 27 (H3K27ac). While H3K27ac marks active enhancers, the detailed chromatin architecture of enhancers remains unclear. Using super-resolution microscopy, we reveal distinct structural states of H3K27ac modified chromatin in response to ERα activation. In estradiol (E2)-treated cells, H3K27ac modified chromatin adopts open, elongated structures associated with active enhancers, while ERα inhibition induces compact, spherical H3K27ac modified chromatin conformations linked to repression. A constitutively active ERα mutation linked to endocrine therapy resistance in breast cancer maintains o

SUBMITTER: Akhshi T 

PROVIDER: S-EPMC12262593 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature