Attomolar Detection of HIV-1 with Label-Free RCA-rCRISPR on Smartphone.

ABSTRACT: Human Immunodeficiency Virus-1 (HIV) remains a major global public health challenge, having led to over 42.3 million deaths since its discovery in the early 1980s. Despite progress in prevention and treatment, around 60% of people with HIV (PWH) remain undiagnosed in resource-limited regions, disproportionately affecting vulnerable populations and underserved communities across the world. This illustrates the critical need for accessible, accurate, and equipment-free diagnostic tools to enhance detection and thus provide opportunities to curb its spread. Here, we developed a low-cost, robust, and label-free rolling circle amplification (RCA)-rCRISPR diagnostic platform for detecting HIV viral load with minimal instrumentation. Our strategy, combining the integration of RNA-detecting RCA re

SUBMITTER: Mohammad N 

PROVIDER: S-EPMC12262608 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature