Surface-Tethering Enhances Precision in Measuring Diffusion Within 3D Protein Condensates.

ABSTRACT: Biomolecular condensates, or membraneless organelles, play pivotal roles in cellular organization by compartmentalizing biochemical reactions and regulating diverse processes such as RNA metabolism, signal transduction, and stress response. Super-resolved imaging and single-molecule tracking are essential for probing the internal dynamics of these condensates, yet intrinsic Brownian (thermal capillary wave) motion of the entire condensate in vitro could introduce artifacts into diffusion measurements, confounding the interpretation of molecular mobility. Here, we systematically assess and address this question using both experiments and simulations. We deploy three surface-tethering strategies-using biotinylated DNA, protein, or antibody tethers-to immobilize FUS protein condensates on pas

SUBMITTER: Sumrall ER 

PROVIDER: S-EPMC12262706 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature