CFIm25-Dependent Alternative Polyadenylation in AKT2 mRNA Programs Macrophage Polarization.

ABSTRACT: Macrophage polarization is essential for immune responses, tissue homeostasis, and progression of many diseases. It is a tightly regulated process involving an intricate network of signaling pathways and control mechanisms at the level of transcription, alternative mRNA splicing, translation and mRNA stability. However, regulation through alternative mRNA polyadenylation (APA), remains poorly understood. This study explores the function of CFIm25, a key APA regulator, in macrophage polarization. Our findings show that CFIm25 overexpression drives M1 polarization, as evident from increased nitric oxide synthase activity, CD80 expression, and pro-inflammatory cytokine secretion, but dampens the M2 phenotype. Conversely, CFIm25 knockdown suppresses M1 traits and promotes M2 characteristics. F

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC12262724 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature