Simultaneous recording of spikes and calcium signals in odor-evoked responses of <i>Drosophila</i> antennal neurons.

ABSTRACT: Most insects, including agricultural pests and disease vectors, rely on olfaction for key innate behaviors. Consequently, there is growing interest in studying insect olfaction to gain insights into odor-driven behavior and to support efforts in vector control. Calcium imaging using GCaMP fluorescence is widely used to identify olfactory receptor neurons (ORNs) responsive to ethologically relevant odors. However, accurate interpretation of GCaMP signals in the antenna requires understanding both response uniformity within an ORN population and how calcium signals relate to spike activity. To address this, we optimized a dual-modality recording method combining single-sensillum electrophysiology and widefield imaging for Drosophila ORNs. Calcium imaging showed that homotypic ab2A neu

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC12262726 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature