Antibiotics Rewire Core Metabolic and Ribosomal Programs in Mammalian Cells.

ABSTRACT: Antibiotics are routinely added to mammalian cell culture media to prevent bacterial growth. However, the use of antibiotics in cell culture can confound downstream experimental results. While genomic and transcriptomic differences between cell cultures treated with and without antibiotics are well-documented, far fewer, if any, comprehensive proteomic comparisons on the use of antibiotics in cell culture have been performed. Here, we present a study on the proteome-wide differences of culturing HepG2 cells in antibiotic (i.e., penicillin/streptomycin) and non-antibiotic-containing media. Using a longitudinal and crossover treatment study design, we analyzed 119 samples across nine passages and four conditions. On average, 9,374 proteins were detected per sample, and we identified 3

SUBMITTER: Movassaghi CS 

PROVIDER: S-EPMC12262799 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature