Chemoenzymatic Synthesis of Asymmetric Bisecting Bi-, Tri-, and Tetra-Antennary <i>N</i>-Glycans.

ABSTRACT: N-Acetylglucosaminyltransferase-III (GnT-III) is a glycosyltransferase that can install a β1,4-linked N-acetylglucosamine (GlcNAc) residue at the central β-mannoside of N-glycans. The resulting so-called bisecting GlcNAc is not further extended by glycosyl transferases and has been implicated a wide range of biological processes. The molecular mechanisms by which bisection modulates the biosynthesis of N-glycans and influences molecular recognition is not well understood, which is due to a lack of well-defined N-glycans with and without bisection. We describe a chemoenzymatic methodology that can readily provide a wide range of asymmetrical bisecting bi-, tri- and tetra-antennary N-glycans. It was found GnT-III can act on bi-, tri- and tetra-antenn

SUBMITTER: Ghotekar BK 

PROVIDER: S-EPMC12262819 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature