Persistent and progressive acute lung allograft dysfunction is linked to cell compositional and transcriptional changes in small airways.

ABSTRACT:

Background

Acute lung allograft dysfunction (ALAD) is a clinical syndrome of forced expiratory volume in 1-second (FEV₁) decline concerning for chronic lung allograft dysfunction (CLAD) onset. Novel diagnostic tools are needed to identify those with ALAD who will progress to CLAD and to target appropriate therapies. We hypothesized that progressive ALAD would be associated with changes in small airway cell composition and cell-specific transcription.

Methods

We prospectively identified recipients with undifferentiated ALAD and controls with stable allograft function for small airway brushing and single-cell RNA sequencing analysis. ALAD outcome group was categorized as (1) control (n = 8), or ALAD with (2) recovered (n = 4), (3) persistent (n = 5), or (4) progress

SUBMITTER: Brunet-Ratnasingham E 

PROVIDER: S-EPMC12262935 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature