The incidence and predictors of false-negative pancreatobiliary fluorescence in situ hybridization (PB-FISH) in biliary strictures: A prospective study.

ABSTRACT:

Background

Fluorescence in situ hybridization (FISH) is recommended as part of multimodality sampling in the evaluation of biliary strictures, but can be false negative in 40%-50% cases. The aim of this study was to comprehensively assess predictors of false-negative FISH in patients with biliary strictures.

Methods

Patients undergoing tissue sampling for biliary strictures from October 20 to February 22 were prospectively enrolled (NCT04572711). Procedural factors such as sampling techniques, order, and utilization of combination sampling were recorded. Results of an optimized pancreatobiliary FISH panel with locus-specific probes for 1q21, 7p12, 8q24, and 9p21 were reported per guidelines. Statistical analyses were performed using BlueSky Statistics v.10.3.1, R package v8.

SUBMITTER: Aggarwal M 

PROVIDER: S-EPMC12262981 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature