Maturation of thymocytes with a monoclonal TCR under control of Trac promoter elements in the absence of β-selection.

ABSTRACT: Thymocyte maturation is a tightly controlled and sequential process of T cell receptor (TCR) gene rearrangement that generates a broad repertoire of T cells with minimal self-reactivity. We previously generated TCR exchange (TRex) mice by targeting a mesothelin-specific "1045" TCR to the Trac locus in murine zygotes. While 1045 T cells from TRex mice display physiological development and function, some T cells coexpress endogenous TCRβ chains, suggesting that β-selection is required for 1045 T cell development. Here, we evaluate thymocyte maturation in the setting of compromised β-selection by deleting endogenous Tcrb or Rag2 in TRex mice. T cells readily form in TRex mice lacking Tcrb, though thymocytes mature through developmental trajectories that appear dependent on interleukin-7 and γ

SUBMITTER: Tsai AK 

PROVIDER: S-EPMC12263106 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature