Integrated single-cell chromatin and transcriptomic analyses of peripheral immune cells in patients with alopecia areata.

ABSTRACT:

Introduction

Alopecia areata (AA) is an autoimmune disorder characterized by non-scarring hair loss ranging from mild, self-limiting episodes to severe and chronic forms. While prior research has primarily focused on lesional skin, the contribution of systemic immune cells remains underexplored.

Methods

We performed integrated single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) on peripheral blood mononuclear cells (PBMCs) from patients with mild and severe AA, as well as healthy controls. A total of 32,453 high-quality cells were analyzed across 36 immune cell subtypes.

Results

In AA patients, especially those with severe disease, we observed increased transcriptional heterogeneity, cytokine and ch

SUBMITTER: Gay-Mimbrera J 

PROVIDER: S-EPMC12263667 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature