Climatic Determinants of Physicochemical Traits and Probiotic Composition in Dwarf Honeybee (<i>Apis florea</i>) Honey.

ABSTRACT: The presence of lactobacilli in honey is highly significant, primarily due to their potential to inhibit pathogens and confer antimicrobial properties. To investigate this, honey samples were collected from 40 Apis florea colonies situated across the southern, southeastern, and southwestern provinces of Iran. Initial screening involved Gram staining and catalase activity analysis of the isolated bacteria. Following genomic DNA extraction from these bacterial colonies, the 16S rRNA genes were amplified via polymerase chain reaction (PCR using LAB-specific primers (27F and 1492R)). This study successfully employed a targeted methodology to isolate 100 lactic acid bacteria (LAB) strains from A. florea honey. Subsequent phylogenetic analysis and phenotypic characterization led to

SUBMITTER: Parichehreh S 

PROVIDER: S-EPMC12264384 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature