AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx.

ABSTRACT: Ion channels in roots allow the plant to gain access to nutrients. The composition of the individual ion channels and the functional contribution of different alpha-subunits is largely unknown. Focusing on K(+)-selective ion channels, we have characterized AtKC1, a new alpha-subunit from the Arabidopsis shaker-like ion channel family. Promoter-beta-glucuronidase (GUS) studies identified AtKC1 expression predominantly in root hairs and root endodermis. Specific antibodies recognized AtKC1 at the plasma membrane. To analyze further the abundance and the functional contribution of the different K(+) channels alpha-subunits in root cells, we performed real-time reverse transcription-PCR and patch-clamp experiments on isolated root hair protoplasts. Studying all shaker-like ion channel alpha-su

SUBMITTER: Reintanz B 

PROVIDER: S-EPMC122651 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature