MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia.

ABSTRACT:

Background

Biliary atresia (BA) is one of the most common liver diseases in infants and young children. Its etiology and pathogenesis are still unclear. The purpose was to investigate the mechanism by which the highly expressed miRNA-29b in exosomes regulates the high expression of platelet-derived growth factor (PDGF) through methylation, hepatic stellate cell activation, and participation in the hepatic fibrosis of BA.

Methods

Children with BA and choledochal cysts (CC) had their peripheral blood exosomes examined in previous studies, and it was discovered that miRNA-29b was substantially expressed in the BA children's exosomes. In contrast, PDGF was highly expressed. Jurkat cells were infected with lentivirus overexpressing miRNA-29b, and the exosomes of Jurkat cells were

SUBMITTER: Liao X 

PROVIDER: S-EPMC12265160 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature