Enhanced anaerobic synthesis of rhamnolipids and emulsification of crude oil by genetically engineered Pseudomonas aeruginosa strains.

ABSTRACT:

Background

Pseudomonas aeruginosa is a facultative anaerobic bacterium widely distributed in oil reservoirs. Efficient production of rhamnolipids by P. aeruginosa in anoxic environments of oil reservoirs contributes to oil displacement. How to enhance the synthesis of rhamnolipids by P. aeruginosa under anaerobic conditions has become a new scientific question.

Results

Pseudomonas aeruginosa SG anaerobically synthesized much lower yield of rhamnolipids than that under aerobic conditions. The key genes rmlBDAC (m), rhlABRI (h) and rhlC (c) and their combination genes were overexpressed in P. aeruginosa. Among seven genetically engineered strains, P. aeruginosa SGhm anaerobically synthesized the highest yield of rhamnolipids, 1.34 g/L, which was about 4.5-fold higher than that

SUBMITTER: Zhao F 

PROVIDER: S-EPMC12265193 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature