Integrated visible-light optical coherence tomography and fluorescence scanning laser ophthalmoscopy to image retinal ganglion cell axons.

ABSTRACT: Retinal ganglion cell (RGC) soma and axonal damage is a hallmark of optic neuropathies. Visible-light OCT fibergraphy (vis-OCTF) enables non-invasive imaging and quantitative assessment of individual RGC axon bundles; however, validating vis-OCTF using confocal fluorescence imaging of flat-mounted postmortem retina is less accurate due to structural alterations caused by flat-mount preparation and cannot be performed longitudinally. For in vivo vis-OCTF validation, we developed an integrated visible-light optical coherence tomography (vis-OCT) and fluorescence scanning laser ophthalmoscopy (SLO) system. The vis-OCT had a 100 nm bandwidth with a center wavelength of 560 nm, offering an axial resolution of 1.3 µm in the retina. The lateral resolutions of vis-OCT and SLO were 4 µm and 3.5 µm,

SUBMITTER: Xu T 

PROVIDER: S-EPMC12265446 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature