Label-free surface sectioning deep ultraviolet tissue imaging in multimodalities.

ABSTRACT: Histology is essential for accurate and effective diagnosis of many diseases and cancers. However, the slicing and staining processes are often expensive, complex, and sometimes invasive to biomedical samples. deep ultraviolet (DUV) microscopy has recently emerged as a promising label-free imaging technique due to the strong absorption of nucleic acids at DUV wavelengths. Here, we present a DUV confocal microscope for label- and slide-free, high-resolution tissue imaging. Compared to visible light, DUV illumination offers thinner optical sectioning and improved contrast due to its shallow penetration depth. We demonstrate that larger pinhole sizes can be used without loss of image quality, enhancing system efficiency. In addition to the DUV confocal microscopy, we explored transmission, ep

SUBMITTER: Chen J 

PROVIDER: S-EPMC12265480 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature