Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic m ⁶ A Regulation in Bovine Preimplantation Development.

ABSTRACT: RNA N ⁶ -methyladenosine (m ⁶ A) plays a crucial role in regulating gene expression during early embryonic development. However, the m ⁶ A dynamics at single-nucleotide resolution in preimplantation development remain uncharacterized, and the functional significance of site specific m ⁶ A modifications in key developmental regulators is largely unknown. Here, using SAC-seq, a single-base resolution, antibody-independent m ⁶ A profiling method, we generate the first comprehensive m ⁶ A landscape in bovine oocytes and preimplantation embryos. We identify a previously uncharacterized m ⁶ A site in RPL12 transcript that is essential for embryonic development. Loss of m ⁶ A at this site leads to reduc

SUBMITTER: Iyyappan R 

PROVIDER: S-EPMC12265534 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature