Using Imaris to rigorously track PET-defined sites of lung inflammation in <i>Mycobacterium tuberculosis</i>-exposed non-human primates.

ABSTRACT: Aerosol exposure of non-human primates (NHPs) to Mycobacterium tuberculosis (Mtb) typically results in discrete sites of inflammation of the lung that is detectable by 2-deoxy-2-[fluorine-18]fluoro-D-glucose (¹⁸F-FDG)-based PET/CT scans. Such scans are often analyzed using software such as Invicro VivoQuant or OsiriX as 3D images by manual labeling sites of PET signal using 2D slices and by reporting maximal SUV either of the whole lung or of individual lesions. Here we propose a pipeline for analysis of the same PET/CT scans using Imaris, a proprietary software typically used for analysis of data from fluorescent microscopy experiments. We show that by using locations of spine vertebra (denoted as "landmarks") we can align serials scans of the same a

SUBMITTER: Hurtado E 

PROVIDER: S-EPMC12265552 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature