Robust regulatory interplay of enhancers, facilitators, and promoters in a native chromatin context.

ABSTRACT: Enhancers are gene-distal cis-regulatory elements that drive cell type-specific gene expression. While significant progress has been made in identifying enhancers and characterizing their epigenomic features, much less effort has been devoted to elucidating mechanistic interactions among clusters of functionally linked regulatory elements within their endogenous chromatin contexts. Here, we developed a novel recombinase-mediated genome rewriting platform and applied our divergent transcription architectural model to understand how a long-range human enhancer confers a remarkable 10,000-fold activation to its target gene, NMU, at its native locus. Our systematic dissection reveals transcription factor synergy at this enhancer and highlights the interplay between a divergently

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC12265581 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature