Deep and Quantitative Proteomic Profiling of Low Volume Mouse Serum Across the Lifespan.

ABSTRACT: Assessing and validating circulating biomarkers is essential for the development of pre-clinical biomarkers that predict biological aging and aging-phenotypes in mice. However, comprehensive proteomics of serum, especially in longitudinal mouse studies, are limited by low volumes of samples. In this study, we develop a workflow for comprehensive and quantitative proteomic analysis of low volume mouse serum and demonstrate its utility and performance in identifying and evaluating key associations with aging phenotypes. Notably, a nanoparticle (NP)-based serum processing workflow coupled to mass spectrometry (MS) increases proteomic coverage by 3 to 6-fold across a range of volumes and provides a quantitative and reproducible (CV < 10%) pipeline for NP-based studies. In a study of 30 mice (a

SUBMITTER: Dey AK 

PROVIDER: S-EPMC12265655 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature