Hydration free energy is a significant predictor of globular protein incorporation into condensates.

ABSTRACT: Membraneless organelles (MLOs) are assemblies of biomolecules, which function without a dividing lipid membrane in a cellular environment. These MLOs, termed biomolecular condensates, are commonly formed by the thermodynamic process of liquid-liquid phase separation (LLPS) and assembly of large numbers of proteins, nucleic acids and co-solvent molecules. Within MLOs, certain biomolecule types are particularly causative of phase separation, and are termed "scaffolds" as they provide the major driving forces for self-assembly. Other molecules that are present in a condensate, but are less causative than the scaffold molecules are termed "clients". Much effort has recently decoded many of the molecular interactions underlying LLPS in search of predicting equilibrium concentrations and materia

SUBMITTER: Anderson S 

PROVIDER: S-EPMC12265688 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature