Circulating Tumor Cells Are Detectable and Independent of PSA and PSMA-PET Metrics in Localized High-Risk and Biochemically Recurrent Prostate Cancer.

ABSTRACT:

Purpose

This study evaluated the feasibility of detecting circulating tumor cells (CTCs) in localized high risk (HR) and biochemically recurrent (BCR) prostate cancer (PCa) patients and examined the correlation between CTCs, serum prostate specific antigen (PSA) levels, 18F-DCFPyL PET parameters and clinical progression.

Methods

Baseline samples were collected from 105 PCa patients enrolled in clinical trial NCT03181867 before 18F-DCFPyL PET/CT imaging. Patients were divided into two cohorts- localized HR and BCR. CTCs were enriched using magnetic EpCAM-PE beads and enumerated by flow cytometry (n=82) or by CD45 depletion followed by EpCAM/PSMA expression analysis by ddPCR (n=23). CTC value of ≥3 CTCs/10 mL blood was established as abnormal using 10 healthy female controls.

SUBMITTER: Rastogi S 

PROVIDER: S-EPMC12265774 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature