Clinical evaluation of the novel digital liquid chip method for anti-dsDNA detection in SLE.

ABSTRACT:

Objective

This study aims to evaluate the diagnostic performance of the digital liquid chip method (DLCM) compared with indirect immunofluorescence (IIF) and chemiluminescent immunoassay (CLIA) for anti-double-stranded DNA (dsDNA) antibody detection in SLE.

Methods

The retrospective study consecutively enrolled 1349 patients, including 698 with SLE and 651 with other autoimmune diseases at Peking Union Medical College Hospital. Anti-dsDNA antibodies were detected using IIF (EUROIMMUN, Luebeck, Germany), CLIA (YHLO, Shenzhen, China) and DLCM (Livzon, Zhuhai, China). The sensitivity, specificity and area under the curve (AUC) of each method and combination were compared at the recommended manufacturer cut-offs. The agreement between methods and the association between antibody

SUBMITTER: Bai Y 

PROVIDER: S-EPMC12265827 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature