Elucidating the role of mycobacteriophage D29-encoded Gp36 in DNA binding and phage gene expression regulation.

ABSTRACT: Bacteriophage infection leading to progeny production in a bacterial host requires timely expression of phage genes that is regulated by various phage- and bacteria-encoded factors. Mycobacteriophage D29, a lytic bacteriophage, is capable of infecting several mycobacterial species, including pathogenic Mycobacterium tuberculosis. Genomic characterization of D29 revealed two distinct promoters present at extreme ends of the genome that govern expression of phage genes. However, D29-derived transcriptional factors that regulate such expression remain largely unexplored. Here, we have characterized D29-encoded Gp36. We show that Gp36 binds to GC-rich direct repeats in sequence-specific manner. Gp36 makes weak homo-oligomer in vitro, with residues I25 and L35 being important for homo-oligomeri

SUBMITTER: Pillai SS 

PROVIDER: S-EPMC12266134 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature