Histone H3 N-terminal recognition by the PHD finger of PHRF1 is required for proper DNA damage response.

ABSTRACT: Plant homeodomain (PHD) fingers are critical effectors of histone post-translational modifications (PTMs), regulating gene expression and genome integrity, and are frequently implicated in human disease. While most PHD fingers recognize unmodified and methylated states of histone H3 lysine 4 (H3K4), the specific functions of many of the over 100 human PHD finger-containing proteins are poorly understood. Here, we present a comprehensive analysis of one such poorly characterized PHD finger-containing protein, PHRF1. Using biochemical, molecular, and cellular approaches, we demonstrate that PHRF1 robustly binds to histone H3, specifically at its N-terminal region. Through integrating RNA-seq and proteomic analyses, we show that PHRF1 regulates transcription and RNA splicing and plays a criti

SUBMITTER: Jain K 

PROVIDER: S-EPMC12266144 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature