Elastin as a Novel Extracellular Matrix From Aberrant HDAC4 Expression in PDGF-BB-Induced Orbital Fibroblasts From Graves' Ophthalmopathy Patients.

ABSTRACT:

Purpose

The purpose of this study was to investigate fibroblast markers and histone deacetylase (HDAC) 4 in orbital tissues and orbital fibroblasts from patients with Graves' ophthalmopathy (GO) and healthy controls.

Methods

Hematoxylin and eosin (H&E), Masson's trichrome, and Verhoeff's Van Gieson (VVG) staining were performed on GO and control orbital tissues. Immunohistochemistry on fibroblast markers were investigated. GO orbital fibroblasts (GOFs) and control orbital fibroblasts (COFs) stimulated with platelet-derived growth factor (PDGF)-BB were assessed for collagen type I alpha I (COL1A1), elastin (ELN), and fibrillin-2 (FBN2) mRNA expression. HDAC4 knockdown in GOF and COF were assessed to study its impact on elastin expression. GO orbital tissues and GOF treated wi

SUBMITTER: Chompoowong R 

PROVIDER: S-EPMC12266294 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature