Proteomic profiling of <i>Escherichia coli</i> cytoplasmic proteins under sublethal boric acid stress.

ABSTRACT:

Background/aim

Boron is an essential micronutrient for plants and certain bacteria, where it plays critical roles in cellular processes at low concentrations. However, elevated levels of boron-containing compounds, such as boric acid, exhibit antimicrobial toxicity. Although the physiological effects of boric acid on bacteria have been partially characterized, its proteome-wide impacts remain poorly elucidated. This study employs a 2D-PAGE-based proteomic approach to investigate how sublethal boric acid stress alters the cytoplasmic proteome of Escherichia coli BW25113.

Materials and methods

E. coli BW25113 cultures were grown to mid-log phase in tryptic soy broth (TSB) and exposed to 70 mM boric acid (a sublethal concentration) or left untreated as a control.

SUBMITTER: Col B 

PROVIDER: S-EPMC12266352 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature